|
KMID : 0368420040470040330
|
|
Journal of Plant Biology
2004 Volume.47 No. 4 p.330 ~ p.337
|
|
|
Defects in a proteolytic step of light-harvesting complex II in an Arabidopsis stay-green mutant,ore10, during dark-induced leaf senescence
|
|
Oh Min-Hyuk
Kim Jin-Hong
Moon Yong-Hwan
Lee Choon-Hwan
|
|
|
Abstract
|
|
|
|
During dark-induced leaf senescence (DIS), the non-functional stay-green mutantore10 showed delayed chlorophyll (Chl) degradation and increased stability in its light-harvesting complex II (LHCII). These phenomena were closely related to the formation of aggregates that mainly consisted of terminal-truncated LHCII (Oh et al., 2003). Theore10 mutant apparently lacks the protease needed to degrade the truncated LHCII. In wild-type (WT) plants, protease was found in the thylakoid fraction, but not the soluble fraction. A similar experiment using dansylated LHCII revealed that the protease degraded both WT andore10 LHCII, indicating that its stability inore10 perhaps did not result from a defect in the LHCII polypeptides themselves. Although protease activity was not present in non-senesced WT leaves, it was induced during DIS. It also was possible to diminish the high level of protease present in the thylakoids through high-salt washing, suggesting that this enzyme is extrinsically bound to the outer surface of the stroma-exposed thylakoid regions.
|
|
|
KEYWORD
|
|
|
Arabidopsis thaliana, leaf senescence, light-harvesting complex II, proteolysis, stay-green mutant
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|